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α cfos 9f6 rabbit mab  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc α cfos 9f6 rabbit mab
    α Cfos 9f6 Rabbit Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1762 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cfos/c-Fos+Rabbit+mAb/pmc13015735-3-0-7
    Average 98 stars, based on 1762 article reviews
    α cfos 9f6 rabbit mab - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Incubation:

    Article Title: Two distinct cell types of the medial mammillary body forming segregated subcircuits
    Article Snippet: Following 24 h of post fixation at 4 °C, brain samples were sectioned at 30 μm using a vibratome (Leica VT1000S), or transferred into 30% sucrose in 0.1 M PB for cryoprotection, embedded in OCT, and sectioned at 30 μm using a cryostat (Leica CM1950). .. Sections were blocked in PBS containing 0.05% Triton and 5% donkey serum and then incubated with the following primary antibodies in the blocking solution at room temperature overnight: RFP (goat polyclonal antibody, 1:2000, SICGEN AB0081-200; rabbit polyclonal antibody, 1:2000, Rockland 600-401-379), GFP (chicken polyclonal antibody, 1:1000, Aves Labs, GFP-1020), PV (mouse monoclonal antibody, 1:1000, Sigma P3088; goat polyclonal antibody, 1:1000, SWant PVG-213), CB (mouse monoclonal antibody, 1:5000, Swant 300; rabbit polyclonal antibody, 1:5000, Swant 38), NeuN (mouse monoclonal antibody, 1:250, Millipore, MAB377), cFos (rabbit polyclonal antibody, 1:500, Cell Signaling Technology 2250). .. Sections were then incubated with appropriate Alexa fluor dye-conjugated IgG secondary antibodies (1:500; Thermofisher Scientific) in blocking solution and mounted in Aqua-mount (LERNER Laboratories).

    Article Title: FRA1 ( FOSL1 ) suppresses neoplastic transformation and modulates radiation responses via transcriptional control of mitogenic and stress-responsive networks
    Article Snippet: .. The blots were washed three times with TBS-T for 5 min each at room temperature, followed by incubation with the following primary rabbit monoclonal antibodies overnight at 4 °C: FRA1 (1:1,000; Cell Signaling #5281T) and cFOS (1:500; Cell Signaling #2250S). .. The blots were washed four times with TBS-T and incubated with HRP-conjugated goat antirabbit secondary antibody (ThermoScientific, G21234 ) at 1:10,000 dilution for 1 h at room temperature.

    Article Title: Neuropeptide Y neurons surrounding the locus coeruleus inhibit noradrenergic system activity to reduce anxiety
    Article Snippet: .. Primary antibodies against NPY (Novus Biologicals, NBP1-46535), TH (LNC1, Millipore, MAB318), GABA (Sigma-Aldrich, A2052), SST (OriGene, AP33464SU-N), ChAT (Millipore, AB144P), cFos (9F6, Cell Signaling Technology, 2250S), and GFP (Aves, GFP-1020) were incubated overnight at 4°C. .. Secondary antibodies (Alexa Fluor 488, 568, or 647, Invitrogen) were incubated for 2 hours (RT), in some cases, followed by 4′,6-diamidino-2-phenylindole (DAPI) nuclear staining (20 min, RT).

    Article Title: Two distinct cell types of the medial mammillary body forming segregated subcircuits.
    Article Snippet: Following 24 h of post fixation at 4 °C, brain samples were sectioned at 30 μm using a vibratome (Leica VT1000S), or transferred into 30% sucrose in 0.1 M PB for cryoprotection, embedded in OCT, and sectioned at 30 μm using a cryostat (Leica CM1950). .. Sections were blocked in PBS containing 0.05% Triton and 5% donkey serum and then incubated with the following primary antibodies in the blocking solution at room temperature overnight: RFP (goat polyclonal antibody, 1:2000, SICGEN AB0081-200; rabbit polyclonal antibody, 1:2000, Rockland 600-401-379), GFP (chicken polyclonal antibody, 1:1000, Aves Labs, GFP-1020), PV (mouse monoclonal antibody, 1:1000, Sigma P3088; goat polyclonal antibody, 1:1000, SWant PVG-213), CB (mouse monoclonal antibody, 1:5000, Swant 300; rabbit polyclonal antibody, 1:5000, Swant 38), NeuN (mouse monoclonal antibody, 1:250, Millipore, MAB377), cFos (rabbit polyclonal antibody, 1:500, Cell Signaling Technology 2250). .. Sections were then incubated with appropriate Alexa fluor dye-conjugated IgG secondary antibodies (1:500; Thermofisher Scientific) in blocking solution and mounted in Aqua-mount (LERNER Laboratories).

    Article Title: Optogenetics-integrated gut organ culture system connects enteric neurons dynamics and gut homeostasis
    Article Snippet: Blocking solution containing 10% donkey serum (Merck, D9663), 5% Bovine serum albumin (BSA) (Millipore,821006), and 0.1% Triton X-100 (Millipore,1086031000) was added for 1 hour at room temperature (RT). .. Primary antibodies anti-βIII-tubulin (Abcam ab41489), cFos (Cell Signaling Technology, 2250), and HuC/D (Santa Cruz SC-515624) were diluted in blocking solution (1:100) and incubated overnight at 4 °C. .. For ChAT staining, primary antibodies against ChAT (Abcam, clone EPR16590 ) were diluted 1:1000 in blocking solution and incubated with the samples for 48 hr at 4 °C.

    Article Title: Optogenetics-integrated gut organ culture system connects enteric neurons dynamics and gut homeostasis.
    Article Snippet: Blocking solution containing 10% donkey serum (Merck, D9663), 5% Bovine serum albumin (BSA) (Millipore,821006), and 0.1% Triton X-100 (Millipore,1086031000) was added for 1 hour at room temperature (RT). .. Primary antibodies anti-βIII-tubulin (Abcam ab41489), cFos (Cell Signaling Technology, 2250), and HuC/D (Santa Cruz SC-515624) were diluted in blocking solution (1:100) and incubated overnight at 4 °C. .. For ChAT staining, primary antibodies against ChAT (Abcam, clone EPR16590) were diluted 1:1000 in blocking solution and incubated with the samples for 48 hr at 4 °C.

    Blocking Assay:

    Article Title: Two distinct cell types of the medial mammillary body forming segregated subcircuits
    Article Snippet: Following 24 h of post fixation at 4 °C, brain samples were sectioned at 30 μm using a vibratome (Leica VT1000S), or transferred into 30% sucrose in 0.1 M PB for cryoprotection, embedded in OCT, and sectioned at 30 μm using a cryostat (Leica CM1950). .. Sections were blocked in PBS containing 0.05% Triton and 5% donkey serum and then incubated with the following primary antibodies in the blocking solution at room temperature overnight: RFP (goat polyclonal antibody, 1:2000, SICGEN AB0081-200; rabbit polyclonal antibody, 1:2000, Rockland 600-401-379), GFP (chicken polyclonal antibody, 1:1000, Aves Labs, GFP-1020), PV (mouse monoclonal antibody, 1:1000, Sigma P3088; goat polyclonal antibody, 1:1000, SWant PVG-213), CB (mouse monoclonal antibody, 1:5000, Swant 300; rabbit polyclonal antibody, 1:5000, Swant 38), NeuN (mouse monoclonal antibody, 1:250, Millipore, MAB377), cFos (rabbit polyclonal antibody, 1:500, Cell Signaling Technology 2250). .. Sections were then incubated with appropriate Alexa fluor dye-conjugated IgG secondary antibodies (1:500; Thermofisher Scientific) in blocking solution and mounted in Aqua-mount (LERNER Laboratories).

    Article Title: Two distinct cell types of the medial mammillary body forming segregated subcircuits.
    Article Snippet: Following 24 h of post fixation at 4 °C, brain samples were sectioned at 30 μm using a vibratome (Leica VT1000S), or transferred into 30% sucrose in 0.1 M PB for cryoprotection, embedded in OCT, and sectioned at 30 μm using a cryostat (Leica CM1950). .. Sections were blocked in PBS containing 0.05% Triton and 5% donkey serum and then incubated with the following primary antibodies in the blocking solution at room temperature overnight: RFP (goat polyclonal antibody, 1:2000, SICGEN AB0081-200; rabbit polyclonal antibody, 1:2000, Rockland 600-401-379), GFP (chicken polyclonal antibody, 1:1000, Aves Labs, GFP-1020), PV (mouse monoclonal antibody, 1:1000, Sigma P3088; goat polyclonal antibody, 1:1000, SWant PVG-213), CB (mouse monoclonal antibody, 1:5000, Swant 300; rabbit polyclonal antibody, 1:5000, Swant 38), NeuN (mouse monoclonal antibody, 1:250, Millipore, MAB377), cFos (rabbit polyclonal antibody, 1:500, Cell Signaling Technology 2250). .. Sections were then incubated with appropriate Alexa fluor dye-conjugated IgG secondary antibodies (1:500; Thermofisher Scientific) in blocking solution and mounted in Aqua-mount (LERNER Laboratories).

    Article Title: Optogenetics-integrated gut organ culture system connects enteric neurons dynamics and gut homeostasis
    Article Snippet: Blocking solution containing 10% donkey serum (Merck, D9663), 5% Bovine serum albumin (BSA) (Millipore,821006), and 0.1% Triton X-100 (Millipore,1086031000) was added for 1 hour at room temperature (RT). .. Primary antibodies anti-βIII-tubulin (Abcam ab41489), cFos (Cell Signaling Technology, 2250), and HuC/D (Santa Cruz SC-515624) were diluted in blocking solution (1:100) and incubated overnight at 4 °C. .. For ChAT staining, primary antibodies against ChAT (Abcam, clone EPR16590 ) were diluted 1:1000 in blocking solution and incubated with the samples for 48 hr at 4 °C.

    Article Title: Optogenetics-integrated gut organ culture system connects enteric neurons dynamics and gut homeostasis.
    Article Snippet: Blocking solution containing 10% donkey serum (Merck, D9663), 5% Bovine serum albumin (BSA) (Millipore,821006), and 0.1% Triton X-100 (Millipore,1086031000) was added for 1 hour at room temperature (RT). .. Primary antibodies anti-βIII-tubulin (Abcam ab41489), cFos (Cell Signaling Technology, 2250), and HuC/D (Santa Cruz SC-515624) were diluted in blocking solution (1:100) and incubated overnight at 4 °C. .. For ChAT staining, primary antibodies against ChAT (Abcam, clone EPR16590) were diluted 1:1000 in blocking solution and incubated with the samples for 48 hr at 4 °C.

    Bioprocessing:

    Article Title: FRA1 ( FOSL1 ) suppresses neoplastic transformation and modulates radiation responses via transcriptional control of mitogenic and stress-responsive networks
    Article Snippet: .. The blots were washed three times with TBS-T for 5 min each at room temperature, followed by incubation with the following primary rabbit monoclonal antibodies overnight at 4 °C: FRA1 (1:1,000; Cell Signaling #5281T) and cFOS (1:500; Cell Signaling #2250S). .. The blots were washed four times with TBS-T and incubated with HRP-conjugated goat antirabbit secondary antibody (ThermoScientific, G21234 ) at 1:10,000 dilution for 1 h at room temperature.

    Chromatin Immunoprecipitation:

    Article Title: Agouti integrates environmental cues to regulate paternal behaviour.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies were against the following: - cFOS (1:2000 dilution, Cell Signalling, monoclonal, Rabbit, 2250S) Monoclonal cFOS antibody was produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala286 of human c-Fos protein. .. - Tyrosine Hydroxylase (1:2000 dilution, ImmunoStar, monoclonal, Mouse, 22941) The Tyrosine Hydroxylase Antibody from ImmunoStar, Inc. is a Mouse Monoclonal antibody to Diap1, DIAPH1, Pale, Th, Tyrosine Hydroxylase, and ple.

    Flow Cytometry:

    Article Title: Agouti integrates environmental cues to regulate paternal behaviour.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies were against the following: - cFOS (1:2000 dilution, Cell Signalling, monoclonal, Rabbit, 2250S) Monoclonal cFOS antibody was produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala286 of human c-Fos protein. .. - Tyrosine Hydroxylase (1:2000 dilution, ImmunoStar, monoclonal, Mouse, 22941) The Tyrosine Hydroxylase Antibody from ImmunoStar, Inc. is a Mouse Monoclonal antibody to Diap1, DIAPH1, Pale, Th, Tyrosine Hydroxylase, and ple.

    Magnetic Resonance Imaging:

    Article Title: Agouti integrates environmental cues to regulate paternal behaviour.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies were against the following: - cFOS (1:2000 dilution, Cell Signalling, monoclonal, Rabbit, 2250S) Monoclonal cFOS antibody was produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala286 of human c-Fos protein. .. - Tyrosine Hydroxylase (1:2000 dilution, ImmunoStar, monoclonal, Mouse, 22941) The Tyrosine Hydroxylase Antibody from ImmunoStar, Inc. is a Mouse Monoclonal antibody to Diap1, DIAPH1, Pale, Th, Tyrosine Hydroxylase, and ple.

    Produced:

    Article Title: Agouti integrates environmental cues to regulate paternal behaviour.
    Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Primary antibodies were against the following: - cFOS (1:2000 dilution, Cell Signalling, monoclonal, Rabbit, 2250S) Monoclonal cFOS antibody was produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala286 of human c-Fos protein. .. - Tyrosine Hydroxylase (1:2000 dilution, ImmunoStar, monoclonal, Mouse, 22941) The Tyrosine Hydroxylase Antibody from ImmunoStar, Inc. is a Mouse Monoclonal antibody to Diap1, DIAPH1, Pale, Th, Tyrosine Hydroxylase, and ple.



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